Semen Wash Medium

Code: GTI-17

Description

For washing of semen during centrifugation prior to in vitro fertilization

Description

The medium contains a very low concentration of Sodium Bicarbonate and is phosphate buffered and must therefore not be CO2 equilibrated

The medium is ready-to-use and does not need any supplements Medium should be preheated to 38.8°C prior to use with the lid on

Key Ingredients:

Calcium Chloride, Magnesium Sulphate, Potassium Chloride, Sodium Bicarbonate, Sodium Chloride, Sodium Dihydrogen Phosphate, Sodium Lactate, Sodium Pyruvate, Phenol Red and Gentamycin

Advantages of using a non-gradient and non-capacitating medium

• The capacitation of the spermatozoa is delayed until they are in the actual IVF medium with the oocytes
• Spermatozoa preserve energy for the subsequent capacitation, acrosome reaction and fertilization
• Potential toxic gradients and excessive centrifugation is avoided
• The Semen Wash Medium also applies very well to sex-sorted semen

Quality Control Tests and Manufacturing

• Sterility tested (Fungal and Bacterial)
• Osmolality tested and pH tested
• Endotoxin tested
• Manufacturer is ISO9001 and ISO13485 certified
• Controlled environment and to human standards
• Bovine Embryo Assay (BEA)
• Batch analysis certificate available

Storage:

• Store in original unopened container refrigerated 3-9 °C and protected from light
• Do not expose to temperatures higher than 39°C
• Do not freeze product it may precipitate < 2°C
• The medium must be used within 10 days after opening, providing it has been
• handled aseptically and is kept refrigerated after opening
• Discard unused medium if it has been warmed