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Obtain higher embryo survival and pregnancy rates with vitrification than after slow freeze
Particularly in vitro and biopsied embryos benefit from this cryopreservation method
Pre-heat media 38.8°C prior to use -leave lid on in order to avoid evaporation
Sucrose, Ficoll, Dimethyl Sulphoxide (DMSO) Ethylene Glycol, Glucose, Sodium Pyruvate, Albumin, Inorganic Salts The cryoprotective ingredients work by dehydrating embryos combined with an influx of high concentrations of cryoprotectants by increasing osmolality stepwise from 290 mOsm/kg to 865 mOsm/kg and the reverse procedure for warming (rehydration of embryo and removal of cryoprotectants) Above steps are essential for rapid cooling into LN2, which takes place from 30 °C to −196 °C in less than one second See the detailed protocol on Vitrification and Warming of embryos
OPU Medium, 500ml
# GTI-500
WASH Medium, 50ml
# GTI-050
IVM Medium, 20ml
# GTI-020
H-IVM Medium, 20ml
# GTI-0020
Semen Wash Medium, 50ml
Sperm Gradient Upper Layer 45%, 20ml
# GTI-45
Sperm Gradient Lower Layer 90%, 20ml
# GTI-90
IVF Medium, 20ml